A photoacoustic imaging system with variable gain at different depths

Video-rate functional photoacoustic microscopy at depths.

We report the development of functional photoacoustic microscopy capable of video-rate high-resolution in vivo imaging in deep tissue. A lightweight photoacoustic probe is made of a single-element broadband ultrasound transducer, a compact photoacoustic beam combiner, and a bright-field light delivery system. Focused broadband ultrasound detection provides a 44-μm lateral resolution and a 28-μm...

Evaluation of Reflection Optical Imaging Characteristics Using Fluorescence with Near Infrared Wavelength at Different Depths of Tissue Equivalent Phantom

A differential photoacoustic imaging system

We describe a differential photoacoustic (PA) imaging system in which almost equal intensity beams modulated 180' out of phase illuminate two regions of a sample in a PA cell. For two regions that are both optically and thermally similar and also homogeneous, the resulting signal is almost zero but if one region has a sub-surface defect this will give an increased signal. This differential sign...

In vivo photoacoustic tomography of chemicals: high-resolution functional and molecular optical imaging at new depths.

High-resolution volumetric optical imaging modalities, such as confocal microscopy, two-photon microscopy, and optical coherence tomography, have become increasingly important in the biomedical imaging field. However, due to strong light scattering, the penetration depths of these imaging modalities are limited to the optical transport mean free path in biological tissues, for example, ∼1 mm in...

simultaneous two - photon calcium imaging at different depths with spatiotemporal multiplexing

nature methods | ADVANCE ONLINE PUBLICATION | Optical probing of neuronal activity with fluorescent calcium indicators is a powerful approach to study information processing in the brain1. In particular, calcium imaging with two-photon laser scanning microscopy (2PLSM)2 is an ideal tool for recording network activity in vivo because it is less invasive than electrophysiology, more cells can be ...